Launch SyncroPatch 384i

Launch of the SyncroPatch 384i

On June 21st 2019 we launched the SyncroPatch 384i – a giga-ohm seal HTS automated patch clamp platform based on the newly introduced and state-of-the art Biomek i5 liquid handler. It provides effortless ion channel screening coupled with unmatched flexibility, ease-of-use and reliability. The SyncroPatch 384i builds on the success of the SyncroPatch 384PE, which has been globally established as the preferred automated patch clamp workhorse in Pharma, Biotech, CRO and academia.

Read the Press Release here.

SyncroPatch 384i Movie

The SyncroPatch 384i is ideal for all of your ion channel screening projects, regardless of whether you are working on complex biophysical properties in an academic lab, or drug screening in industry. Now large compound screens on ion channels are no problem. Watch the video here. 

SyncroPatch 384i Brochure

Fifteen years ago we introduced our first automated patch clamp system. Over the years both our portfolio and technology have constantly improved. We have listened to the needs of our customers in order to offer the best possible innovative instruments for APC and beyond.  Read the brochure here

SyncroPatch 384i Webpage

The new SyncroPatch 384i builds on the success of the SyncroPatch 384PE. Now we further improved flexibility, ease-of-use and reliability. Learn about the benefits and new features of the SyncroPatch 384/768i. Visit our product page here.
User Meeting, Nano Innovation Award, Diversity

2019 Nano Innovation Award

Nanion Technologies congratulates the winners of the Nano Innovation Awards: Thomas Hümmer (Ludwig-Maximilians-Universität München) & Sabrina Thomä (University of Bayreuth) who received the €9,000 Bavarian-wide prize.
Nanion Technologies is proud to be an annual sponsor of the Nano Innovation Awards.
Read more here.

Three new Instrument Launches in September

September will be busy: We are launching three new instruments! 
One new instrument is the FLEXcyte 96 (an add-on of the CardioExcyte 96) which analyzes the contraction force of iPSC-derived cardio-myocytes on flexible membranes.
The new FLEXcyte 96 product page was published recently.
Visit the FLEXcyte product page here.

Latest Publications

SyncroPatch 384PE:
Chemical Synthesis, Proper Folding, Nav Channel Selectivity Profile and Analgesic Properties of the Spider Peptide Phlotoxin 1.
Nicolas S. et al., Toxins (2019) - Download here.

SyncroPatch 384PE:
Predicting Functional Effects of Missense Variants in Voltage-Gated Sodium and Calcium Channels.
Heyne H.O. et al., bioRxiv (2019) - Download here.

SyncroPatch 384PE:
Functional consequences of a KCNT1 variant associated with status dystonicus and early‐onset infantile encephalopathys.
Gertler T.S. et al., (2019) Annals of Clinical and Translational Neurology - Download here.

SyncroPatch 384PE:
In vitro and in vivo characterization of a synthetic scorpion toxin AmmTx3, a potent inhibitor of cardiac voltage-gated potassium channel Kv4.2.
Nicolas S. et al., (2019) Archives of Cardiovascular Diseases Supplements - Download here.

Control of Lysosomal TRPML1 Channel Activity and Exosome Release by Acid Ceramidase in Mouse Podocytes.
Li G. et al., (2019) American Journal of Physiology Cell Physiology - Download here.

CardioExcyte 96:
Transcriptomic profiling reveals p53 as a key regulator of doxorubicin-induced cardiotoxicity.
McSweeney K.M. et al., (2019) Cell Death Discovery - Download here.

Orbit 16:
A comparison of ion channel current blockades caused by individual poly(ethylene glycol) molecules and polyoxometalate nanoclusters.
Wang H. et al., (2019) The European Physical Journal E - Download here.

Orbit mini:
Arg-8 of yeast subunit e contributes to the stability of F-ATP synthase dimersand to the generation of the full-conductance megachannel.
Guo L. et al., (2019) Journal of Biological Chemistry - Download here.

Vesicle Prep Pro:
Linker length in fluorophore–cholesterol conjugates directs phase selectivity and cellular localisation in GUVs and live cells.
O' Connor D. et al., (2019) RSC Advances - Download here.

Vesicle Prep Pro:
Cellular uptake of self-assembled phytantriol-based hexosomes is independent of major endocytic machineries.
Rodrigues L. et al., (2019) Journal of Colloid Interface Science - Download here.

10th User Meeting Munich

Welcome to our Annual User Meeting 2019. It will be held in October 17. - 18 in Munich at the headquarters of Nanion.
You are cordially invited to join us! We are expecting approximately 80 attendees from industry and academia.
The User Meeting includes a symposium, a poster presentation session and an instrument demonstration.
Visit our registration page here.

We love Diversity at Nanion

With a workforce comprised of over 20 nationalities, we at Nanion are proud to embrace diversity within our personnel. In today’s era of globalization, diversity is not only significant but very fundamental for the growth of our company. We initiated a series of employee interviews to introduce our international colleagues, starting with Meghana Bhat Subray who moved from India to Germany to work in our IT department.
Read Meghana's interview here.

Mark Your Calendar

28. August - 11. September 2019:
Microelectrode Techniques For Cell Physiology
(Plymouth, United Kingdom). Meet András Horváth.

04. - 09. September 2019:
SGP 73rd Annual Symposium and SOBLA Annual Meeting
(Valparaiso, Chile). Meet Andrea Brüggemann.

08. - 11. September 2019:
Eurotox 2019
(Helsinki, Finland). Meet Elena Dragicevic.

08. - 11. September 2019:
The 30th Meeting of the Association Canaux Ionique
(Sète, France). Meet Conrad Weichbrodt.

12. - 14. September 2019:
International KV7 Channels Symposium
(Naples, Italy). Meet András Horváth.

Nanion Day out

19th July 2019: We (team Nanion Munich) had a fantastic time at the Weingut Ilmbacher Hof in Iphofen. After a very detailed and entertaining vineyard tour, we had food and wine tasting that was tailored to perfection. We had an amazing time, lots of laughs and tasted more wine than expected!
Find more pictures on our facebook page here.

Snapshot of the Month

"Don't be upsetti, eat some spaghetti". A snapshot of Nanion staff enjoying lunch out on the terrace located at our headquarters in Munich. (Left to right) Sonja Stölzle-Feix, Niels Fertig, Jürgen Steindl, Frank Henrichsen, Alison Obergrussberger and Claudia Haarmann.
The calcium and sodium activated potassium channels: KCa & KNa
The KCa and KNa family: history, structure and function 

Although it could be demonstrated in the 1950's that elevations in intracelllular Ca2+ increased K+ permeability in red blood cells, it wasn't until the 1990's that the first Ca2+ activated K+ channels were identified in drosophila and mammals. The first channel was named KCa1.1, also known as BK and Slo1, and the functional channel comprises 4 pore-forming a subunits as a tetramer. Genes encoding other KCa channels were subsequently identified, given the names KCa2.1 (also known as SK1), KCa2.2 (SK2), KCa2.3 (SK3) and KCa3.1 (also known as SK4 or IK). Other genes encoding KCa4.1 (Slack, Slo2.2), KCa4.2 (Slick, Slo2.1) and KCa5.1 (SLO3) were also assigned to the family, although over the years it became evident that KCa4.1 and KCa4.2 are regulated by Na+ and not Ca2+ and so have been renamed KNa1.1 and KNa1.2, respectively. The KCa and KNa channels underlie many important physiological functions including regulation of neuronal excitability and action potential firing, maintaining K+ homeostasis and cell volume regulation. Mutations in KNa1.1 underlie early onset epilepsy disorders and mutations or differences in expression levels of KCa1.1 have been linked to clinical conditions such as hypertension, diabetes, asthma and epilepsy.
Mutation in KNa1.1 causes early onset infantile epilepsy

In a paper published recently, both manual patch clamp and the SyncroPatch 768PE were used to identify a mutation in the KCNT1 gene which encodes the KNa1.1 ion channel. When expressed in CHO cells the mutation (L437F) exhibited a gain-of-function phenotype. The SyncroPatch 768PE was used to screen different anticonvulsant compounds on KCNT1-L437F mutant currents. Only quinidine was found to block the current with the other compounds causing no significant change in current amplitude. The use of automated patch clamp offers the potential to investigate not only FDA-approved anticonvulsants, but also repurposed drugs and new rationally designed compounds to find novel therapeutics for conditions thus poorly treated by currently available therapeutics.

Read the full paper here and find out more about Al George Jr. and his research here.

KCa and KNa Recommended Assays, Cell Lines and Literature

KCa3.1, KCa1.1 and KNa1.1 expressed in cell lines or primary cells can be recorded using APC instruments with internal exchange/perfusion

Recommended assays:
  • SyncroPatch 384PE (HTS patch clamp): external solution exchange; internal perfusion; temperature control
  • Patchliner (automated patch clamp): external solution exchange; internal solution exchange; temperature control; dynamic clamp
  • Port-a-Patch(automated patch clamp): external perfusion; internal perfusion; temperature control)

Recommended cell lines:

Read KCa3.1 Application Note and KNa1.1 paper

Visit our website for more Application Notes!
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